aN. D. Zelinsky Institute of Organic
Chemistry, Russian Academy of Sciences, Moscow, Russia
bCenter of Microbiology and Virology, Polish Academy of Sciences, Lodz, Poland
KEYWORDS: O-antigen, bacetrial polysaccharide, D-galacturonic acid, Ne-(1-carboxyethyl)lysine, structure, NMR spectroscopy, Proteus mirabilis.
Biochemistry/Moscow, 1997, v. 62(5), pp. 509-513,
An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium Proteus mirabilis O13 and found to contain D-galactose, 2-acetamido-2-deoxy-D-glucose, and Ne-(1-carboxyethyl)-N*-(D-galacturonoyl)lysine. On the basis of full acid hydrolysis and 1H- and 13C-NMR spectroscopy, including two-dimensional correlation spectroscopy (COSY), H-detected heteronuclear 1H,13C multi-quantum coherence (HMQC), and rotating-frame nuclear Overhauser effect spectroscopy (ROESY), the following structure of the branched trisaccharide repeating unit of the polysaccharide was established:
Ne-(1-carboxyEt)Lys(N-6)aDGalpA |4 -3)bDGlcpNAc(1-3)aDGalp(1-