aN. D. Zelinsky Institute of Organic
Chemistry, Russian Academy of Sciences, Moscow, Russia
bCenter of Microbiology and Virology, Polish Academy of Sciences, Lodz, Poland
KEYWORDS: Lipopolysaccharide; O-Antigen structure; Bacterial acidic polysaccharide; Hexuronic acid; Lysine, L-; (Proteus mirabilis)
FEBS Lett., 1996, v. 386, pp. 247-251
The polysaccharide chains of the Proteus mirabilis serogroups O26 and O30 lipopolysaccharides were studied using sugar and methylation analysis and 1H and 13C NMR spectroscopy, including two-dimensional correlation spectroscopy and rotating-frame NOE spectroscopy. The polysaccharides were found to be acidic due to the presence of D-galacturonic acid and its amide with L-lysine in serogroup O26 or D-glucuronic acid in serogroup O30, and the structures of their tetrasaccharide repeating units were established. The O26-specific polysaccharide is structurally and serologically related to the O-specific polysaccharide of P. mirabilis O28, which includes amides of DGalA with L-lysine and L-serine [Radziejewska-Lebrecht, J. et al. (1995) Eur. J. Biochem. 230, 705-712].