aN. D. Zelinsky Institute of Organic
Chemistry, Russian Academy of Sciences, Moscow, Russia
bCenter of Microbiology and Virology, Polish Academy of Sciences, Lodz, Poland
KEYWORDS: lipopolysaccharide, O-antigen, polysaccharide, structure, NMR spectroscopy, Proteus mirabilis
Biochemistry/Moscow, 1996, v. 61(5), pp. 575-579
Based on acid hydrolysis, methylation, and one- and two-dimensional 1H- and 13C-NMR spectroscopy, including homonuclear and 1H,13C heteronuclear correlation spectroscopy (COSY) and rotating-frame nuclear Overhauser effect spectroscopy (ROESY), it was found that the O-specific polysaccharide chain of the lipopolysaccharide of the bacterium Proteus mirabilis O30 is a hexosaminoglucuronan built up of tetrasaccharide repeating units having the following structure:
The degree of O-acetylation of 2-acetamido-2-deoxyglucose is about 70%.