aDepartment of General Microbiology, Institute of Microbiology and Immunology, University of Lodz, Poland
bN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
KEYWORDS: Bacterial polysaccharide, O-Antigen, Lipopolysaccharide, Proteus penneri, NMR spectroscopy, serological cross-reactivity
European J. Biochem., 2000, v. 267, pp. 808-814
DOI: 10.1046/j.1432-1327.2000.01059.x (free full text)
A neutral O-specific polysaccharide (O-antigen) was isolated from the lipopolysaccharide (LPS) of the bacterium Proteus penneri 71. On the basis of sugar analysis and 1H 13C NMR spectroscopic studies including two-dimensional COSY, 1H,13C heteronuclear COSY and ROESY, the following structure of the trisaccharide repeating unit of the polysaccharide was established:
The polysaccharide has the same carbohydrate backbone as the O-specific polysacccharide of P. penneri 19 and both are similar to that of P. penneri 62 studied by us previously. A cross-reactivity of anti-P. penneri 71, 19 and 62 O-antisera with 11 P. penneri strains was revealed and substantiated at the level of the O-antigen structures. These strains could be divided into three subgroups within a new proposed Proteus O64 serogroup containing P. penneri strains only.