STRUCTURE OF THE O-SPECIFIC POLYSACCHARIDE OF PROTEUS PENNERI 71 AND CLASSIFICATION OF CROSS-REACTIVE P. PENNERI STRAINS TO A NEW PROPOSED SEROGROUP O64

K. Zycha, N.A. Kocharovab, M. Kowalczyka, F.V. Toukachb, D. Kaminskaa, A.S. Shashkovb, Y.A. Knirelb, Z. Sidorczyka

aDepartment of General Microbiology, Institute of Microbiology and Immunology, University of Lodz, Poland
bN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia

KEYWORDS: Bacterial polysaccharide, O-Antigen, Lipopolysaccharide, Proteus penneri, NMR spectroscopy, serological cross-reactivity

European J. Biochem., 2000, v. 267, pp. 808-814

DOI: 10.1046/j.1432-1327.2000.01059.x    (free full text)


A neutral O-specific polysaccharide (O-antigen) was isolated from the lipopolysaccharide (LPS) of the bacterium Proteus penneri 71. On the basis of sugar analysis and 1H 13C NMR spectroscopic studies including two-dimensional COSY, 1H,13C heteronuclear COSY and ROESY, the following structure of the trisaccharide repeating unit of the polysaccharide was established:

-3)bDGlcpNAc(1-4)bDGlcpNAc(1-3)aDGalp(1-

The polysaccharide has the same carbohydrate backbone as the O-specific polysacccharide of P. penneri 19 and both are similar to that of P. penneri 62 studied by us previously. A cross-reactivity of anti-P. penneri 71, 19 and 62 O-antisera with 11 P. penneri strains was revealed and substantiated at the level of the O-antigen structures. These strains could be divided into three subgroups within a new proposed Proteus O64 serogroup containing P. penneri strains only.


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