N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
KEYWORDS: Providencia alcalifaciens; 2,4-dihydroxypentanoic acid; sugar ether; bacterial polysaccharide structure
Natural Product Communications, 2008, v. 3(10), pp. 1625-1630
New components of bacterial polysaccharides, (2S,4R)- and (2R,4R)-2,4-dihydroxypentanoic acids, were identified using two-dimensional 1H and 13C NMR spectroscopy in the O-antigens of Providencia alcalifaciens O8 and O31. The acids were ether-linked via their 2-HO-group to a residue of N-acetylglucosamine or mannose at position 4. The absolute configurations of the acids were determined by NOE spectroscopy combined with molecular modeling.