aN. D. Zelinsky Institute of Organic
Chemistry, Russian Academy of Sciences, Moscow, Russia
bCenter of Microbiology and Virology, Polish Academy of Sciences, Lodz, Poland
KEYWORDS: Proteus vulgaris, O-antigen, O-specific polysaccharide, lipopolysaccharide, O-acetylation, 3-acetamido-3,6-dideoxy-D-glucose
Carbohydrate Research, 1999, v. 318(1-4), pp. 146-153
The acidic O-specific polysaccharide of Proteus vulgaris O22 was studied using 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, NOESY, and H-detected 1H,13C heteronuclear multiple-quantum coherence (HMQC) experiments, and the following structure of the branched pentasaccharide repeating unit was established:
65% 2-OAc, 4-OAc-aDQuip3NAc |3 -3)bDGlcpNAc(1-2)bLRhap(1-4)aLRhap(1-4)bDGlcpA(1-
where Qui3NAc is 3-acetamido-3,6-dideoxyglucose, O-acetylation of QuiNAc at position 4 is stoichiometric and at position 2 nonstoichiometric. Serological relationships of P. vulgaris O22 with some other Proteus strains was substantiated on the level of the O-antigen structures.