STRUCTURAL AND SEROLOGICAL STUDIES OF THE O-SPECIFIC POLYSACCHARIDE OF THE BACTERIUM PROTEUS MIRABILIS O10 CONTAINING L-ALTRURONIC ACID, A NEW COMPONENT OF O-ANTIGENS

A.S. Swierzkoa, A.S. Shashkovb, S.N. Senchenkovab, F.V. Toukachb, A. Ziolkowskia, M. Cedzynskia, N.A. Paramonovb, W. Kacaa, Y.A. Knirelb*

aCenter of Microbiology and Virology, Polish Academy of Sciences, Lodz, Poland
bN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia

KEYWORDS: lipopolysaccharide, O-antigen, polysaccharide, uronic acids, structure, NMR spectroscopy, Proteus mirabilis

FEBS Lett., 1996, v. 398, pp. 297-302
Also in Biochemistry/Moscow, 1996, v. 61(9), pp. 1100-1105

DOI: 10.1016/S0014-5793(96)01061-7


An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium Proteus mirabilis O10 and studied by full acid hydrolysis before and after carboxyl reduction and by 1H- and 13C-NMR spectroscopy, including two-dimensional correlation spectroscopy (COSY), H-detected heteronuclear 1H,13C multi-quantum coherence (HMQC), and rotating-frame nuclear Overhauser effect spectroscopy (ROESY). It was found that the O-specific polysaccharide contains one residue each of 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose, D-galacturonic acid, and L-altruronic acid, and the following structure of the branched tetrasaccharide repeating unit of the polysaccharide was esteblished:

   aLAltpA
      |3
-4)aDGalpNAc(1-3)aDGalpA(1-3)aDGlcpNAc(1-

ScienceНаука : Proteus ScienceПубликации : Proteus ScienceДом : Наука