THE O-POLYSACCHARIDE FROM THE LIPOPOLYSACCHARIDE OF PROVIDENCIA STUARTII O44 CONTAINING L-QUINOVOSE, A 6-DEOXY SUGAR RARELY UCCURRING IN BACTERIAL POLYSACCHARIDES

Nina A. Kocharovaa, Olga G. Ovchinnikovaa*, Filip V. Toukacha, Agnieszka Torzewskab, Alexander S. Shashkova, Yuriy A. Knirela and Antoni Rozalskib

aN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
bDepartment of Immunobiology of Bacteria, Institute of Microbiology and Immunology, University of Lodz, Poland

KEYWORDS: Providencia stuartii, O-antigen, Lipopolysaccharide, Polysaccharide structure

Carbohydrate Research, 2005, v. 340, pp. 1419-1423

DOI: 10.1016/j.carres.2005.02.020


The O-polysaccharide (O-antigen) of Providencia stuartii O44:H4 (strain 3768/51) was obtained by mild acid degradation of the lipopolysaccharide and studied by sugar and methylation analyses along with 1H and 13C NMR spectroscopy, including 2D 1H,1H COSY, TOCSY, ROESY and H-detected 1H,13C HSQC and HMQC-TOCSY experiments. The O-polysaccharide was found to have a branched hexasaccharide repeating unit of the following structure:

    bDGlcpA
       |4
->3)aLFucp(1->3)aDGlcp(1->4)aLQuip(1->3)aDGlcpNAc(1->4)aDGalpNAc(1->

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