THE STRUCTURE OF THE O-POLYSACCHARIDE FROM THE LIPOPOLYSACCHARIDE OF PROVIDENCIA STUARTII O57 CONTAINING AN AMIDE OF D-GALACTURONIC ACID WITH L-ALANINE

Nina A. Kocharova^a, Olga G. Ovchinnikova^a*, Ivan S. Bushmarinov^a, Filip V. Toukach^a, Agnieszka Torzewska^b, Alexander S. Shashkov^a, Yuriy A. Knirel^a and Antoni Rozalski^b

^aN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
^bDepartment of Immunobiology of Bacteria, Institute of Microbiology and Immunology, University of Lodz, Poland

KEYWORDS: Providencia stuartii, O-antigen, Lipopolysaccharide, Polysaccharide structure

Carbohydrate Research, 2005, v. 340, pp. 775-780

DOI: 10.1016/j.carres.2005.01.007

The O-polysaccharide (O-antigen) was obtained by mild acid degradation of the lipopolysaccharide of Providencia stuartii O57:H29. Studies by sugar and methylation analyses along with ¹H and ¹³C NMR spectroscopy, including two-dimensional ¹H,¹H COSY, TOCSY, ROESY, H-detected ¹H,¹³C HSQC, and HMBC experiments, showed that the polysaccharide contains an amide of D-galacturonic acid with L-alanine and has the following pentasaccharide repeating unit:

   75% OAc                  L-Ala
       |2                      |6
->3)aLRhap(1->4)aDGlcp(1->4)aDGalpA(1->3)bDGlcpNAc(1->2)aDGalp(1->

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