STRUCTURE OF THE ACIDIC O-SPECIFIC POLYSACCHARIDE OF THE BACTERIUM PROTEUS MIRABILIS O10 CONTAINING L-ALTRURONIC ACID, A NEW COMPONENT OF O-ANTIGENS

A.S. Shashkova, S.N. Senchenkovaa, F.V. Toukacha, A. Ziolkowskib, N.A. Paramonova, W. Kacab, Y.A. Knirela*, N.K. Kochetkova

aN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
bCenter of Microbiology and Virology, Polish Academy of Sciences, Lodz, Poland

KEYWORDS: lipopolysaccharide, O-antigen, polysaccharide, uronic acids, structure, NMR spectroscopy, Proteus mirabilis

Biochemistry/Moscow, 1996, v. 61(9), pp. 1100-1105
Also in FEBS Lett., 1996, v. 398, pp. 297-302


An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium Proteus mirabilis O10 and studied by full acid hydrolysis before and after carboxyl reduction and by 1H- and 13C-NMR spectroscopy, including two-dimensional correlation spectroscopy (COSY), H-detected heteronuclear 1H,13C multi-quantum coherence (HMQC), and rotating-frame nuclear Overhauser effect spectroscopy (ROESY). It was found that the O-specific polysaccharide contains one residue each of 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose, D-galacturonic acid, and L-altruronic acid, and the following structure of the branched tetrasaccharide repeating unit of the polysaccharide was esteblished:

   aLAltpA
     |3
-4)aDGalpNAc(1-3)aDGalpA(1-3)aDGlcpNAc(1-

ScienceScience: Proteus SciencePublications: Proteus ScienceHome : Science