STRUCTURE OF THE O-SPECIFIC POLYSACCHARIDE OF THE BACTERIUM PROTEUS MIRABILIS O13 CONTAINING A NOVEL AMIDE OF D-GALACTURONIC ACID WITH Ne-(1-CARBOXYETHYL)LYSINE

A.S. Shashkova, F.V. Toukacha, S.N. Senchenkovaa, A. Ziolkowskib, N.A. Paramonova, W. Kacab, Y.A. Knirela*, N.K. Kochetkova

aN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
bCenter of Microbiology and Virology, Polish Academy of Sciences, Lodz, Poland

KEYWORDS: O-antigen, bacetrial polysaccharide, D-galacturonic acid, Ne-(1-carboxyethyl)lysine, structure, NMR spectroscopy, Proteus mirabilis.

Biochemistry/Moscow, 1997, v. 62(5), pp. 509-513,


An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium Proteus mirabilis O13 and found to contain D-galactose, 2-acetamido-2-deoxy-D-glucose, and Ne-(1-carboxyethyl)-N*-(D-galacturonoyl)lysine. On the basis of full acid hydrolysis and 1H- and 13C-NMR spectroscopy, including two-dimensional correlation spectroscopy (COSY), H-detected heteronuclear 1H,13C multi-quantum coherence (HMQC), and rotating-frame nuclear Overhauser effect spectroscopy (ROESY), the following structure of the branched trisaccharide repeating unit of the polysaccharide was established:

Ne-(1-carboxyEt)Lys(N-6)aDGalpA
                          |4
       -3)bDGlcpNAc(1-3)aDGalp(1-

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