STRUCTURE OF THE NEW ACIDIC O-ANTIGEN OF PROTEUS VULGARIS O22 CONTAINING O-ACETYLATED 3-ACETAMIDO-3,6-DIDEOXY-D-GLUCOSE

F.V. Toukacha, B. Bartodziejskab, S.N. Senchenkovaa, M. Wykrotab, A.S. Shashkova, A. Rozalskib, Y.A. Knirela

aN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
bCenter of Microbiology and Virology, Polish Academy of Sciences, Lodz, Poland

KEYWORDS: Proteus vulgaris, O-antigen, O-specific polysaccharide, lipopolysaccharide, O-acetylation, 3-acetamido-3,6-dideoxy-D-glucose

Carbohydrate Research, 1999, v. 318(1-4), pp. 146-153

DOI: 10.1016/S0008-6215(99)00088-9


The acidic O-specific polysaccharide of Proteus vulgaris O22 was studied using 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, NOESY, and H-detected 1H,13C heteronuclear multiple-quantum coherence (HMQC) experiments, and the following structure of the branched pentasaccharide repeating unit was established:

65% 2-OAc, 4-OAc-aDQuip3NAc
                   |3
-3)bDGlcpNAc(1-2)bLRhap(1-4)aLRhap(1-4)bDGlcpA(1-

where Qui3NAc is 3-acetamido-3,6-dideoxyglucose, O-acetylation of QuiNAc at position 4 is stoichiometric and at position 2 nonstoichiometric. Serological relationships of P. vulgaris O22 with some other Proteus strains was substantiated on the level of the O-antigen structures.


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