Other research

For the recent 20 years I've been working in the N.D. Zelinsky Institute of Organic Chemistry NMR center as glycopolymer structural researcher in the group of Prof. Knirel (partly as a phd student supervised by Prof. Shashkov). Our systematic chemical and immunochemical studies are aimed at correlation between the O-antigen structure and the immunospecificity of strains, which is important for obtaining synthetic diagnosticums, vaccines and immunostimulators.

Besedes a bacterial class on which I focused in 1996-2002 (Proteus studies), a lot of other bacterial O-antigens were investigated (see below).

The other projects whichg I participated or carried out personally:

Glyco-studies included the elucidation of structure of natural glycopolymers and glycoconjugates by NMR spectroscopic methods. Click here to view the approximate plan of a glycopolymer NMR investigation. The NMR experiments were carried out on Bruker NMR instruments (WM250, AM300, DRX500) and included the following:

NMR experiment Its result
1D 13C NMR preliminary data, glycosylation effects (=> substitution pattern, absolute configurations)
1D 1H NMR HH-coupling constants analysis (=> monomeric composition, anomeric configurations)
1D 31P NMR revealing non-sugar substituents
1D H-Gated 13C NMR anomeric configuration, cycle sizes
1D Attached Proton Test methylen groups assignment
2D 1H,1H COSY vicinal proton-proton interactions (=> 1H NMR spectrum assignment)
2D 1H,1H Double Quantum Filter COSY simplifying the COSY spectrum by the removal of diagonal line
2D H,H-relayed COSY (RCT, RCT2) far proton-proton interactions (=> 1H NMR spectrum assignment)
2D TOtal Correlation SpectroscopY excluding the spin system subsets (of residues) from 1H NMR spectrum
1D 1H difference mode double resonance signal shape analysis (=> thorough 1H NMR spectrum assignment )
2D 31P,1H COSY localization of phospho-containing substituents
2D 13C,1H COSY direct heteronuclear coupling constants (=> 13C NMR spectrum assignment)
2D 1H,13C Heteronuclear Multiple-Quantum Coherence direct heteronuclear coupling constants (=> 13C NMR spectrum assignment, constant measurement)
2D Heteronuclear Multiple-Bond Correlation far heteronuclear interactions, including transglycosidic (=> residue sequence)
2D NOE SpectroscopY revealing spatial H-H contacts in oligomers, including transglycosydic (=> substitution pattern, residue sequence)
2D Rotating frame nuclear Overhauser Effect SpectroscopY revealing spatial H-H contacts, including transglycosydic (=> substitution pattern, residue sequence)
1D difference mode NOE spectroscopy thorough analysis of H-H spatial contacts

Structural studies of the Proteus O-antigens were supported by more than 10 RFBR and INTAS grants (grant list here).

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