NEW STRUCTURES OF THE O-SPECIFIC POLYSACCHARIDES OF PROTEUS. Part 3: POLYSACCHARIDES CONTAINING NON-SUGAR ORGANIC ACIDS

A.N. Kondakova^a, F.V. Toukach^a, S.N. Senchenkova^a, N.P. Arbatsky^a, A.S. Shashkov^a, Y.A. Knirel^a, K. Zych^b, A. Torziewska^b, K. Kolodziejska^b, A. Rozalski^b, Z. Sidorczyk^b

^aN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
^bInstitute of Microbiology and Immunology, University of Lodz, Poland

KEYWORDS: lipopolysaccharide, O-antigen, bacterial polysaccharides, structure, Proteus, lactic acid, aminoacids

Biochemistry / Moscow, 2002, v.68, pp.446-457

The classification of Proteus bacteria is based on the structure and immunospecificity of polysaccharides. Four new O-specific polysaccharides of Proteus bacteria were obtained by mild acid hydrolysis off lipopolysaccharides of the following strains: P. myxofaciens, P. vulgaris O44, P. mirabilis G1 (O3) and P. penneri 28. They were found to contain non-sugar organic acids, including lactic acid and aminoacids attached via amide bridge.

The structures were elucidated using NMR spectroscopy, including one-dimensional ¹H and ¹³C NMR spectroscopy, two-dimensional ¹H,¹H correlation (COSY, TOCSY), H-detected ¹H,¹³C heteronuclear multiple-quantum coherence (HMQC) and nuclear Overhauser effect spectroscopy (NOESY), along with chemical methods. The structural data obtained are useful as the chemical basis for the creation of the classification scheme for Proteus strains.

Наука : Proteus

Публикации : Proteus

Дом : Наука