STRUCTURE OF A NEW LYSINE-CONTAINING O-SPECIFIC POLYSACCHARIDE OF THE BACTERIUM PROTEUS MIRABILIS O26

A.S. Shashkov^a, F.V. Toukach^a, N.A. Paramonov^a, A. Ziolkowski^b, S.N. Senchenkova^a, W. Kaca^b, Y.A. Knirel^a*

^aN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
^bCenter of Microbiology and Virology, Polish Academy of Sciences, Lodz, Poland

KEYWORDS: lipopolysaccharide, O-antigen, polysaccharide, L-lysine, structure, NMR spectroscopy, Proteus mirabilis

Biochemistry/Moscow, 1996, v. 61(1), pp. 15-21

Composition and structure of the O-specific polysaccharide chain of the lipopolysaccharide of the bacterium Proteus mirabilis O26 were studied using one- and two-dimensional ¹H- and ¹³C-NMR spectroscopy, including homonuclear correlation spectroscopy (COSY), H-detected ¹H,¹³C heteronuclear multi-quantum coherence, and rotating-frame nuclear Overhauser effect spectroscopy. It was found that the polysaccharide is acidic due to the presence of residues of D-galacturonic acid (D-GalA), part of which are O-acetylated and the other part form an amide with the a-amino group of L-lysine, and is built up of tetrasaccharide repeating units having the following structure:

   L-Lys
     |6
-4)aDGalpA(1-4)aDGalp(1-3)[AcO-4]bDGalpA(1-3)bDGlcpNAc(1-

The P. mirabilis O26 polysaccharide is structurally similar to the O-specific polysaccharide of P. mirabilis O28, which was studied by us earlier and found to contain amides of D-galacturonic acid with L-lysine and L-serine [Eur. J. Biochem. 230, 705-712 (1995)].

Наука : Proteus

Публикации : Proteus

Дом : Наука