STRUCTURE ELUCIDATION AND GENE CLUSTER CHARACTERIZATION OF THE O-ANTIGEN OF YERSINIA KRISTENSENII C-134

O.V. Sizovaa, A.S. Shashkova, Ph.V. Toukacha, Y.A. Knirela, R.Z. Shaikhutdinovab, S.A. Ivanova, A.A. Kislichkinab, S.V. Dentovskayab*

aN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia
bState Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia

KEYWORDS: Yersinia kristensenii; O-specific polysaccharide; O-antigen; Lipopolysaccharide; Glycerol 1-phosphate; Bacterial polysaccharide structure

Carbohydrate Research, 2019, т. 481, стр. 9-15

DOI: 10.1016/j.carres.2019.06.001, PMID: 31220629


Mild acid degradation of the lipopolysaccharide of Yersinia kristensenii C-134 afforded a glycerol teichoic acid-like O-polysaccharide, which was studied by sugar analysis, O-deacetylation and dephosphorylation along with 1D and 2D NMR spectroscopy. The following structure of the O-polysaccharide was established:

      Ac       a-D-Glcp-(1-6)-a-D-Galp(1        a-D-Glcp(1
      |3                               |3                |6
-2)-D-gro-1P-(O-6)-b-D-Glcp-(1-4)-a-L-FucpNAc-(1-3)-b-D-GlcpNAc-(1
This structure is related to those of other Y. kristensenii O-polysaccharides studied earlier. The O-antigen gene cluster of Y. kristensenii C-134 was analyzed and found to be consistent with the O-polysaccharide structure established.


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